Abstract: TLC mesh column chromatography is a new typed column chromao-graphy, which is based on the principles of high performance liquid chromatography. It shows several advantages over conventional column chromatography, i.e. high speed, high resolution, simplicity of equipment and easy operation. It is available to ordinary laboratories. Detailed informations such as laboratory device, packing materials, eluants, column fillings and injection methods are given in this paper.