沉积物中16种多环芳烃单体碳同位素GC-C-IRMS测定

陆燕; 王小云; 曹建平

doi:10.11781/sysydz201804532

沉积物中16种多环芳烃单体碳同位素GC-C-IRMS测定

doi: 10.11781/sysydz201804532

广东石油化工学院, 广东茂名 525000

基金项目:

广东省普通高校特色创新项目（650465）、茂名市科技计划项目（2014038）和广东石油化工学院人才引进项目（513036）资助。

详细信息

作者简介:
陆燕(1978-),女,博士,副教授,从事环境污染微生物作用机制研究。E-mail:hyss.yanlu@163.com。

通讯作者:
曹建平(1963-),男,高级工程师,从事气体稳定同位素分析测试工作。E-mail:caojp_iso@163.com。

中图分类号: TE135
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出版历程
- 收稿日期: 2018-03-10
- 修回日期: 2018-05-24
- 刊出日期: 2018-07-28

Compound-specific carbon stable isotope analysis of 16 polycyclic aromatic hydrocarbons in sediments by Gas Chromatography-Combustion-Isotope Ratio Mass Spectrometry (GC-C-IRMS)

Guangdong University of Petrochemical Technology, Maoming, Guangdong 525000, China

摘要

摘要: 为监测海洋沉积物中的多环芳烃（PAHs）、追踪污染物来源并控制污染，建立了沉积物中16种多环芳烃单体碳同位素GC-C-IRMS测定方法。首先采用EA-IRMS联用技术对16种多环芳烃单体碳同位素进行了定值，偏差范围在0.03‰~0.15‰（n=10），小于0.2‰；再用该16种多环芳烃混标，运用GC-C-IRMS联用技术考察了高效薄层色谱、气相色谱进样体积、无分流时间、进样浓度等可能产生的同位素分馏，结果表明，整个方法并无明显的同位素分馏现象。根据不同浓度PAHs混标在GC-C-IRMS测定中的变化与EA-IRMS测定的差，建立了数据校正曲线，其R²大于0.99，并应用于沉积物中16种多环芳烃单体碳同位素的测定，测试数据为海域沉积物中多环芳烃溯源提供了有力证据。
- EA-IRMS /
- GC-C-IRMS /
- 高效薄层色谱 /
- 单体碳同位素 /
- 多环芳烃 /
- 沉积物
Abstract: A method for the compound-specific carbon stable isotope analysis of 16 polycyclic aromatic hydrocarbons (PAHs) in ocean sediments by Gas Chromatography-Combustion-Isotope Ratio Mass Spectrometry (GC-C-IRMS) was established for monitoring PAHs, tracing pollutant sources and controlling pollution. The compound-specific carbon stable isotope of 16 PAHs was determined by Elementary Analyzer-Isotope Ratio Mass Spectrometry (EA-IRMS) with a peak area deviation of 0.03‰-0.15‰ (n=10) (<0.2‰). The isotope fractionation of the 16 PAHs caused by High Performance Thin Layer Chromatography (HPTLC), injection volume of GC, purge time or sampling concentration was investigated using GC-C-IRMS and PAH-MIX. The results indicated that there was no apparent isotope fractionation during the whole process of analysis. Some calibration curves were obtained according to the changes of GC-C-IRMS data corresponding to the concentrations of PAH-MIX and the differences between GC-C-IRMS and EA-IRMS data, and the R² value was higher than 0.99. The curves were used to determine the compound-specific carbon stable isotope of 16 PAHs in sediments, which offers strong evidence for tracing the source of PAHs in ocean sediments.

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