基于高通量测序技术研究页岩气区上方微生物多样性

顾磊; 许科伟; 汤玉平; 杨帆; 王国建; 杜伟; 高波; 腾格尔

doi:10.11781/sysydz202003443

基于高通量测序技术研究页岩气区上方微生物多样性

doi: 10.11781/sysydz202003443

顾磊^1,,
许科伟^1, ,,
汤玉平¹,
杨帆¹,
王国建¹,
杜伟²,
高波²,
腾格尔^{1, 3}

1.
中国石化石油勘探开发研究院无锡石油地质研究所, 无锡 214126
2.
中国石化石油勘探开发研究院非常规资源研究所, 北京 100083
3.
中国地质调查局油气资源调查中心, 北京 100083

基金项目:

国家科技重大专项 Z017ZX05036-002

国家自然科学基金 U1663202

国家自然科学基金 41690133

国家自然科学基金 41872126

详细信息

作者简介:
顾磊(1987—)，男，博士，副研究员，从事油气微生物勘探开发研究。E-mail：gulei.syky@sinopec.com

通讯作者:
许科伟(1982—)，男，博士，高级工程师，从事油气微生物勘探开发研究。E-mail：xukw.syky@sinopec.com

中图分类号: TE132.2
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出版历程
- 收稿日期: 2020-01-27
- 修回日期: 2020-04-29
- 刊出日期: 2020-05-28

Microbial diversity above a shale gas field using high-throughput sequencing

GU Lei^1
,,
XU Kewei^{1
, ,},
TANG Yuping¹,
YANG Fan¹,
WANG Guojian¹,
DU Wei²,
GAO Bo²,
BORJIGIN Tenger^{1, 3}

1.
Wuxi Research Institute of Petroleum Geology, SINOPEC, Wuxi, Jiangsu 214126, China
2.
Research Institute of Unconventional Resources, SINOPEC Petroleum Exploration & Production Research Institute, Beijing 100083, China
3.
Oil and Gas Survey, China Geological Survey, Beijing 100083, China

摘要

摘要: 微生物油气勘探技术近年来已成为油气地表勘探技术中新兴的勘探检测技术。为进一步了解油气田上方的微生物信息，对页岩气区上方微生物多样性进行了研究。采用高通量测序技术研究了川东南丁山地区油气微生物物种丰度、群落多样性等特征。鉴定出了3种主要油气微生物：甲基嗜氧反硝化菌（Methylophilales）、甲基孢囊菌（Methylocystis）、甲基球菌（Methylococcus），值得进一步做生化研究，可以作为潜在油气微生物指示菌。3种微生物发育丰度与丁山地区逸散区域分布具有较好的匹配关系。高通量解析技术能够在丁山地区油气微生物研究中有很好的应用，得出了基岩样品中微生物分布的特征，并与焦石坝地区油气微生物进行了初步的差异性分析，鉴定出的潜在油气指示菌值得进一步研究。
- 油气微生物勘探 /
- 高通量测序 /
- 群落结构 /
- 微生物多样性
Abstract: In recent years, microbial analysis has become a new detection technology in oil and gas surface exploration. The microbial diversity above a shale gas field was studied in order to further understand microbial information. High-throughput sequencing technology was used to study the abundance and diversity of hydrocarbon species in a shale gas field in the Dingshan area of the southeastern Sichuan Basin. Three main microorganisms were identified, including Methylophilales, Methylocystis, and Methylococcus, which are worthy of further biochemical research and can be used as potential oil and gas indicating bacteria. The developmental abundance of three kinds of microorganisms has a good matching relationship with the distribution of the fugitive area in Dingshan. The high-throughput analytical technology can be applied well in the study of oil and gas microorganisms in Dingshan area, and the distribution characteristics of microorganisms in bedrock samples were obtained. Furthermore, a preliminary analysis of the differences between oil and gas microbes in Dingshan and Jiaoshiba areas was made, indicating that the identified potential oil and gas indicating bacteria deserve further study.
- microbial exploration for oil and gas /
- high-throughput sequencing /
- community structure /
- microbial diversity

HTML全文

图 1 川东南丁山和焦石坝剖面样品采集部署

Figure 1. Sample distribution in Dingshan and Jiaoshiba areas, southeastern Sichuan Basin

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图 2 川东南丁山与焦石坝地区页岩富集区微生物属水平物种的相对丰度

Figure 2. Relative abundance at genus level of microbes in shale gas fields in Dingshan and Jiaoshiba areas, southeastern Sichuan Basin

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图 3 川东南丁山与焦石坝地区页岩富集区物种Rank-Abundance曲线

Figure 3. Species rank vs. relative abundance in shale gas fields in Dingshan and Jiaoshiba areas, southeastern Sichuan Basin

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图 4 川东南丁山与焦石坝地区物种属水平丰度聚热图

Figure 4. Heat distribution at genus level of microbes in shale gas fields in Dingshan and Jiaoshiba areas, southeastern Sichuan Basin

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图 5 川东南丁山地区上方土壤中甲基类油气微生物分布

剖面SS’位置见图 1a

Figure 5. Distribution of methyl hydrocarbons in soil above Dingshan area, southeastern Sichuan Basin

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图 6 川东南焦石坝地区上方土壤中甲基类油气微生物分布

剖面EE’位置见图 1b

Figure 6. Distribution of methyl hydrocarbons in soil above Jiaoshiba area, southeastern Sichuan Basin

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图 7 川东南丁山地区页岩气逸散模式^[21]

Figure 7. Shale gas dissipation model in Dingshan area, southeastern Sichuan Basin

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图 8 川东南丁山地区联井剖面热释烃甲烷浓度分布

剖面SS’位置见图 1a

Figure 8. Concentration of pyrolysis hydrocarbon methane in Dingshan area, southeastern Sichuan Basin

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