Abstract: Forty-three samples of kerogen and inclusions in coals from two basins in China were studied for organic petrology.By combined use of transmissible, reflective lights and fluorescence, the quantification of macerals, the determination of fluorescence spectra and vitrinite reflectance,plates for the relations between fluorescence parameters and vitrinite reflectances were accomplished. The results show that there are some defects in the methods of determing kerogen types by current indexes. Amorphous-dominated kerogen may belong to any chemical type, while its hydrogen enrichment depends on the source of organics, the sedimentary environments and early diagenesis. The fluorescence method can be used to distinguish amorphous kerogen enriched hydrogen from that of poor hydrogen effectively, and to establish the relation between optical classification and element analysis for kerogen) besides, some kerogen samples have very low values of vitrinite reflectance, being unable to reflect organic maturity. But fluorescence parameters can make some remedies about this defects of R°and develop relational plates between fluorescence parameters and R°, and infer the values of vitrinite relectance.