Microbial characteristics of soil in low-amplitude structures in Yuqi area, Tarim Basin
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摘要: 为研究塔里木盆地于奇油气田土壤中微生物的多样性和群落结构特征，采用16S rRNA高通量测序技术，测试了于奇YQ5井东部的油气已知区(YQ-y)和YQ12井西部的油气目标区(YQ-wz)土壤中微生物16S rRNA V4区序列，比较分析了两个区块微生物的群落结构和多样性。YQ-y和YQ-wz两组样品绝大部分的微生物类群具有一致性。α和β多样性分析均表明，YQ-y和YQ-wz两组样品在物种丰度、多样性以及群落结构等方面具有很大的相似性。同时，解析了YQ-y和YQ-wz两组样品属水平排名前10的细菌，发现噬甲基菌属、拟杆菌属、类诺卡氏菌属和芽孢杆菌属是可利用油气藏兼性生长的常见微生物，而且两组样品中均发现大量的未知细菌，说明两组样品都蕴含了丰富的未知微生物资源。基于高通量测序分析，获得的于奇地区油气微生物多样性信息，YQ-y和YQ-wz两组样品整体上微生物类群非常接近，并初步探讨了于奇东部微生物异常点。
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关键词:
- 16S rRNA测序 /
- 微生物多样性 /
- 群落结构 /
- 于奇地区 /
- 塔里木盆地
Abstract: High-throughput sequencing of microbial 16S rRNA gene has been conducted to explore microbial diversity and community structure in the Yuqi oil and gas reservoir sites of Tarim Basin. Soil samples were collected from Yuqi oil and gas reservoir sites (YQ-y) to the east of well YQ 5 and prospecting areas (YQ-wz) to the west of well YQ 12. Most of the two groups of sample have similar microbial community. Both α- and β-diversity analyses show that YQ-y and YQ-wz samples have great similarity in species abundance, diversity and community structure. Ten major hydrocarbon-oxidizing bacteria were identified in the YQ-y and YQ-wz samples, including Methylophaga, Bacteroides, Nocardioides, and Bacillus. Moreover, a large number of unknown bacteria were noticed, indicating that both of the samples contain abundant unknown microbial resources. Oil and gas microbial diversity in the Yuqi area was obtained through high-throughput sequencing. The two groups of samples from YQ-y and YQ-wz areas have similar microbial community. Preliminary discussion on microbial anomaly in the eastern part of Yuqi was also carried out.-
Key words:
- 16S rRNA sequencing /
- bacterial diversity /
- community structure /
- Yuqi area /
- Tarim Basin
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图 2 塔里木盆地于奇地区土壤样品中微生物OTU韦恩图
Figure 2. Venn diagram of OTU numbers based on 16SrRNA gene sequence in Yuqi soil samples, Tarim Basin
图 3 塔里木盆地于奇地区土壤样品YQ-y和YQ-wz两组样品α多样性指数
Figure 3. α diversity of soil samples from YQ-y and YQ-wz, Yuqi area, Tarim Basin
图 4 塔里木盆地于奇地区土壤样品中细菌群落主成分分析
Figure 4. PCA analysis of bacterial community structures in Yuqi soil samples, Tarim Basin
图 5 塔里木盆地于奇地区样品微生物群落组成非度量多维尺度分析
Figure 5. NMDS analysis of bacterial community structures in Yuqi soil samples, Tarim Basin
图 6 塔里木盆地于奇地区土壤样本中门水平物种相对丰度
Figure 6. Distribution of relative abundance of top 10 phyla in Yuqi soil samples, Tarim Basin
图 7 塔里木盆地于奇地区YQ-y和YQ-wz两组土壤样品中属水平物种相对丰度
Figure 7. Distribution of relative abundance of top 10 genus in YQ-y and YQ-wz samples, Yuqi area, Tarim Basin
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