Microbial characteristics of soil in low-amplitude structures in Yuqi area, Tarim Basin
-
摘要: 为研究塔里木盆地于奇油气田土壤中微生物的多样性和群落结构特征，采用16S rRNA高通量测序技术，测试了于奇YQ5井东部的油气已知区(YQ-y)和YQ12井西部的油气目标区(YQ-wz)土壤中微生物16S rRNA V4区序列，比较分析了两个区块微生物的群落结构和多样性。YQ-y和YQ-wz两组样品绝大部分的微生物类群具有一致性。α和β多样性分析均表明，YQ-y和YQ-wz两组样品在物种丰度、多样性以及群落结构等方面具有很大的相似性。同时，解析了YQ-y和YQ-wz两组样品属水平排名前10的细菌，发现噬甲基菌属、拟杆菌属、类诺卡氏菌属和芽孢杆菌属是可利用油气藏兼性生长的常见微生物，而且两组样品中均发现大量的未知细菌，说明两组样品都蕴含了丰富的未知微生物资源。基于高通量测序分析，获得的于奇地区油气微生物多样性信息，YQ-y和YQ-wz两组样品整体上微生物类群非常接近，并初步探讨了于奇东部微生物异常点。
-
关键词:
- 16S rRNA测序 /
- 微生物多样性 /
- 群落结构 /
- 于奇地区 /
- 塔里木盆地
Abstract: High-throughput sequencing of microbial 16S rRNA gene has been conducted to explore microbial diversity and community structure in the Yuqi oil and gas reservoir sites of Tarim Basin. Soil samples were collected from Yuqi oil and gas reservoir sites (YQ-y) to the east of well YQ 5 and prospecting areas (YQ-wz) to the west of well YQ 12. Most of the two groups of sample have similar microbial community. Both α- and β-diversity analyses show that YQ-y and YQ-wz samples have great similarity in species abundance, diversity and community structure. Ten major hydrocarbon-oxidizing bacteria were identified in the YQ-y and YQ-wz samples, including Methylophaga, Bacteroides, Nocardioides, and Bacillus. Moreover, a large number of unknown bacteria were noticed, indicating that both of the samples contain abundant unknown microbial resources. Oil and gas microbial diversity in the Yuqi area was obtained through high-throughput sequencing. The two groups of samples from YQ-y and YQ-wz areas have similar microbial community. Preliminary discussion on microbial anomaly in the eastern part of Yuqi was also carried out.-
Key words:
- 16S rRNA sequencing /
- bacterial diversity /
- community structure /
- Yuqi area /
- Tarim Basin
-
图 2 塔里木盆地于奇地区土壤样品中微生物OTU韦恩图
Figure 2. Venn diagram of OTU numbers based on 16SrRNA gene sequence in Yuqi soil samples, Tarim Basin
图 3 塔里木盆地于奇地区土壤样品YQ-y和YQ-wz两组样品α多样性指数
Figure 3. α diversity of soil samples from YQ-y and YQ-wz, Yuqi area, Tarim Basin
图 4 塔里木盆地于奇地区土壤样品中细菌群落主成分分析
Figure 4. PCA analysis of bacterial community structures in Yuqi soil samples, Tarim Basin
图 5 塔里木盆地于奇地区样品微生物群落组成非度量多维尺度分析
Figure 5. NMDS analysis of bacterial community structures in Yuqi soil samples, Tarim Basin
图 6 塔里木盆地于奇地区土壤样本中门水平物种相对丰度
Figure 6. Distribution of relative abundance of top 10 phyla in Yuqi soil samples, Tarim Basin
图 7 塔里木盆地于奇地区YQ-y和YQ-wz两组土壤样品中属水平物种相对丰度
Figure 7. Distribution of relative abundance of top 10 genus in YQ-y and YQ-wz samples, Yuqi area, Tarim Basin
-
[1] 汤玉平, 顾磊, 许科伟, 等. 油气微生物勘探机理及应用[J]. 微生物学通报, 2016, 43(11): 2386-2395. https://www.cnki.com.cn/Article/CJFDTOTAL-WSWT201611008.htmTANG Yuping, GU Lei, XU Kewei, et al. Research and application of microbial exploration for oil and gas[J]. Microbiology China, 2016, 43(11): 2386-2395. https://www.cnki.com.cn/Article/CJFDTOTAL-WSWT201611008.htm [2] 汤玉平, 许科伟, 顾磊, 等. 油气微生物勘探理论与技术研究进展[J]. 石油实验地质, 2021, 43(2): 325-334. doi: 10.11781/sysydz202102325TANG Yuping, XU Kewei, GU Lei, et al. Recent progress in the theory and technology of microbial prospecting for oil and gas[J]. Petroleum Geology & Experiment, 2021, 43(2): 325-334. doi: 10.11781/sysydz202102325 [3] SINGH N K, CHOUDHARY S. Bacterial and archaeal diversity in oil fields and reservoirs and their potential role in hydrocarbon recovery and bioprospecting[J]. Environmental Science and Pollution Research, 2021, 28(42): 58819-58836. doi: 10.1007/s11356-020-11705-z [4] 梅海, 林壬子, 梅博文, 等. 油气微生物检测技术: 理论、实践和应用前景[J]. 天然气地球科学, 2008, 19(6): 888-893. https://www.cnki.com.cn/Article/CJFDTOTAL-TDKX200806026.htmMEI Hai, LIN Renzi, MEI Bowen, et al. Microbial oil-gas detection technologies: theory, practice and application prospect[J]. Natural Gas Geoscience, 2008, 19(6): 888-893. https://www.cnki.com.cn/Article/CJFDTOTAL-TDKX200806026.htm [5] REDMOND M C, VALENTINE D L, SESSIONS A L. Identification of novel methane-, ethane-, and propane-oxidizing bacteria at marine hydrocarbon seeps by stable isotope probing[J]. Applied and Environmental Microbiology, 2010, 76(19): 6412-6422. doi: 10.1128/AEM.00271-10 [6] 林军章, 汪卫东, 胡婧, 等. 胜利油田微生物采油技术研究与应用进展[J]. 油气地质与采收率, 2021, 28(2): 18-26. https://www.cnki.com.cn/Article/CJFDTOTAL-YQCS202102004.htmLIN Junzhang, WANG Weidong, HU Jing, et al. Progress in research and application of microbial enhanced oil recovery technology in Shengli Oilfield[J]. Petroleum Geology and Recovery Efficiency, 2021, 28(2): 10-17. https://www.cnki.com.cn/Article/CJFDTOTAL-YQCS202102004.htm [7] 曹军, 周进松, 银晓, 等. 微生物地球化学勘探技术在黄土塬地貌区油气勘探中的应用[J]. 特种油气藏, 2020, 27(5): 53-60. https://www.cnki.com.cn/Article/CJFDTOTAL-TZCZ202005008.htmCAO Jun, ZHOU Jinsong, YIN Xiao, et al. Application of microbial geochemical exploration technology in oil and gas exploration in Loess Tableland regions[J]. Special oil & Gas Reservoirs, 2020, 27(5): 53-60. https://www.cnki.com.cn/Article/CJFDTOTAL-TZCZ202005008.htm [8] 顾磊, 许科伟, 汤玉平, 等. 基于高通量测序技术研究页岩气区上方微生物多样性[J]. 石油实验地质, 2020, 42(3): 443-450. doi: 10.11781/sysydz202003443GU Lei, XU Kewei, TANG Yuping, et al. Microbial diversity above a shale gas field using high-throughput sequencing[J]. Petroleum Geology & Experiment, 2020, 42(3): 443-450. doi: 10.11781/sysydz202003443 [9] 温静, 肖传敏, 郭斐, 等. 高凝油油藏微生物驱提高采收率实验研究[J]. 特种油气藏, 2020, 27(6): 127-132. https://www.cnki.com.cn/Article/CJFDTOTAL-TZCZ202301012.htmWEN Jing, XIAO Chuanmin, GUO Fei, et al. Experimental research on microbial flooding technology for enhancing oil recovery in high-pour-point reservoirs[J]. Special Oil & Gas Reservoirs, 2020, 27(6): 127-132. https://www.cnki.com.cn/Article/CJFDTOTAL-TZCZ202301012.htm [10] 汪卫东. 微生物采油技术研究进展与发展趋势[J]. 油气地质与采收率, 2021, 28(2): 1-9. https://www.cnki.com.cn/Article/CJFDTOTAL-YQCS202102001.htmWANG Weidong. Research advance and development trend in microbial enhanced oil recovery technology[J]. Petroleum Geology and Recovery Efficiency, 2021, 28(2): 1-9. https://www.cnki.com.cn/Article/CJFDTOTAL-YQCS202102001.htm [11] 侯兆伟, 李蔚, 乐建君, 等. 大庆油田微生物采油技术研究及应用[J]. 油气地质与采收率, 2021, 28(2): 10-17. https://www.cnki.com.cn/Article/CJFDTOTAL-YQCS202102002.htmHOU Zhaowei, LI Wei, LE Jianjun, et al. Research and application of microbial enhanced oil recovery technology in Daqing Oilfield[J]. Petroleum Geology and Recovery Efficiency, 2021, 28(2): 10-17. https://www.cnki.com.cn/Article/CJFDTOTAL-YQCS202102002.htm [12] 刘雅慈, 何泽, 张胜, 等. 油气田土壤甲烷氧化菌实时荧光定量PCR检测技术的建立与应用[J]. 微生物学通报, 2014, 41(6): 1071-1081. https://www.cnki.com.cn/Article/CJFDTOTAL-WSWT201406006.htmLIU Yaci, HE Ze, ZHANG Sheng, et al. Development and application of a fluorescent quantitative real-time PCR technique for detection of methane-oxidizing bacteria in oil and gas field soil[J]. Microbiology China, 2014, 41(6): 1071-1081. https://www.cnki.com.cn/Article/CJFDTOTAL-WSWT201406006.htm [13] DENG Yue, DENG Chunping, YANG Jinshui, et al. Novel butane-oxidizing bacteria and diversity of bmoX genes in Puguang gas field[J]. Frontiers in Microbiology, 2018, 9: 1576. [14] 张春林, 庞雄奇, 梅海, 等. 微生物油气勘探技术在岩性气藏勘探中的应用: 以柴达木盆地三湖坳陷为例[J]. 石油勘探与开发, 2010, 37(3): 310-315. https://www.cnki.com.cn/Article/CJFDTOTAL-SKYK201003009.htmZHANG Chunlin, PANG Xiongqi, Mei Hai, et al. Application of microbial oil surveying to exploration of lithologic gas reservoirs: a case from the Sanhu Depression, Qaidam Basin, NW China[J]. Petroleum Exploration And Development, 2010, 37(3): 310-315. https://www.cnki.com.cn/Article/CJFDTOTAL-SKYK201003009.htm [15] 张敏, 何泽, 杜建军, 等. 苏干湖盆地连续电磁剖面和微生物基因定量油气勘探[J]. 地质与勘探, 2017, 53(6): 1197-1207. https://www.cnki.com.cn/Article/CJFDTOTAL-DZKT201706016.htmZHANG Min, HE Ze, DU Jianjun, et al. Application of continuous electromagnetic profiling and quantitative detection of gene technique to oil and gas exploration in the Sugan Lake Basin[J]. Geology and Exploration, 2017, 53(6): 1197-1207. https://www.cnki.com.cn/Article/CJFDTOTAL-DZKT201706016.htm [16] 杨帆, 沈忠民, 汤玉平, 等. 准噶尔盆地春光探区油气微生物指示[J]. 石油学报, 2017, 38(7): 804-812. https://www.cnki.com.cn/Article/CJFDTOTAL-SYXB201707007.htmYANG Fan, SHEN Zhongmin, TANG Yuping, et al. Hydrocarbon microbial prospecting in Chunguang exploration area, Junggar Basin[J]. Acta Petrolei Sinica, 2017, 38(7): 804-812. https://www.cnki.com.cn/Article/CJFDTOTAL-SYXB201707007.htm [17] 顾磊, 许科伟, 汤玉平, 等. 基于高通量测序技术研究玉北油田上方微生物多样性[J]. 应用与环境生物学报, 2017, 23(2): 276-282. https://www.cnki.com.cn/Article/CJFDTOTAL-YYHS201702015.htmGU Lei, XU Kewei, TANG Yuping, et al. Microbial diversity in Yubei Oil field determined by high-throughput sequencing[J]. Chinese Journal of Applied & Environmental Biology, 2017, 23(2): 276-282. https://www.cnki.com.cn/Article/CJFDTOTAL-YYHS201702015.htm [18] 廖志勇, 康仁东, 曹远志. 于奇地区中生界油气成藏条件及聚集规律[J]. 石油实验地质, 2016, 38(S1): 83-86. doi: 10.11781/sysydz2016S1083LIAO Zhiyong, KANG Rendong, CAO Yuanzhi. Reservoir formation conditions and accumulation laws in Mesozoic in Yuqi area[J]. Petroleum Geology & Experiment, 2016, 38(S1): 83-86. doi: 10.11781/sysydz2016S1083 [19] 韩勇, 徐浩. 塔河油田于奇地区断裂体系特征研究[J]. 石油地质与工程, 2020, 34(3): 7-12. https://www.cnki.com.cn/Article/CJFDTOTAL-SYHN202003003.htmHAN Yong, XU Hao. Characteristics of fault system in Yuqi area of Tahe oilfield[J]. Petroleum Geology and Engineering, 2020, 34(3): 7-12. https://www.cnki.com.cn/Article/CJFDTOTAL-SYHN202003003.htm [20] 旷理雄, 郭建华, 黄太柱, 等. 塔里木盆地于奇地区奥陶系碳酸盐岩成藏条件及成藏模式[J]. 石油勘探与开发, 2007, 34(3): 299-303. https://www.cnki.com.cn/Article/CJFDTOTAL-SKYK200703007.htmKUANG Lixiong, GUO Jianhua, HUANG Taizhu, et al. Petroleum accumulation in Ordovician carbonate rocks of Yuqi region in Akekule uplift, Tarim Basin[J]. Petroleum Exploration and Development, 2007, 34(3): 299-303. https://www.cnki.com.cn/Article/CJFDTOTAL-SKYK200703007.htm [21] 闫亮, 季苗, 贾宝迁, 等. 塔里木盆地顺北断溶体油气藏微生物特征及有利区预测[J]. 石油与天然气地质, 2020, 41(3): 576-585. https://www.cnki.com.cn/Article/CJFDTOTAL-SYYT202003014.htmYAN Liang, JI Miao, JIA Baoqian, et al. Microbial characteristics of Shunbei faulted-karst reservoirs and prediction of play fairways, Tarim Basin[J]. Oil & Gas Geology, 2020, 41(3): 576-585. https://www.cnki.com.cn/Article/CJFDTOTAL-SYYT202003014.htm [22] 闫亮, 贾宝迁, 季苗, 等. 塔里木盆地新和地区低幅度构造油气微生物特征及有利目标区预测[J]. 石油实验地质, 2020, 42(6): 1001-1008. doi: 10.11781/sysydz2020061001YAN Liang, JIA Baoqian, JI Miao, et al. Microbial characteristics of low-amplitude structures and prediction of favorable target areas in Xinhe area, Tarim Basin[J]. Petroleum Geology & Experiment, 2020, 42(6): 1001-1008. doi: 10.11781/sysydz2020061001 [23] CAPORASO J G, KUCZYNSKI J, STOMBAUGH J, et al. QⅡME allows analysis of high-throughput community sequencing data[J]. Nature Methods, 2010, 7(5): 335-336. [24] BOLYEN E, RIDEOUT J R, DILLON M R, et al. Reproducible, interactive, scalable and extensible microbiome data science using QⅡME 2[J]. Nature Biotechnology, 2019, 37(8): 852-857. [25] ONDOV B D, BERGMAN N H, PHILLIPPY A M. Interactive metagenomic visualization in a web browser[J]. BMC Bioinformatics, 2011, 12(1): 385. [26] SCHLOSS P D, WESTCOTT S L, RYABIN T, et al. Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities[J]. Applied and Environmental Microbiology, 2009, 75(23): 7537-7541. [27] QUAST C, PRUESSE E, YILMAZ P, et al. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools[J]. Nucleic Acids Research, 2013, 41(Database issue): D590-D596. [28] EDGAR R C. UPARSE: highly accurate OTU sequences from microbial amplicon reads[J]. Nature Methods, 2013, 10(10): 996-998. [29] 张继伟. 基于主成分分析的页岩油有利区评价: 以仪陇—平昌地区大安寨段为例[J]. 断块油气田, 2021, 28(1): 28-32. https://www.cnki.com.cn/Article/CJFDTOTAL-DKYT202101007.htmZHANG Jiwei. Evaluation of favorable areas of shale oil based on principal component analysis: taking Daanzhai member of Yilong-Pingchang area as an example[J]. Fault-Block Oil and Gas Field, 2021, 28(1): 28-32. https://www.cnki.com.cn/Article/CJFDTOTAL-DKYT202101007.htm -
下载:
图(8)
计量
- 文章访问数: 426
- HTML全文浏览量: 127
- PDF下载量: 43
- 被引次数: 0
苏公网安备32021102000780号