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Volume 42 Issue 3
May  2020
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Article Navigation >  PETROLEUM GEOLOGY & EXPERIMENT  > 2020  >  42(3): 443-450
GU Lei, XU Kewei, TANG Yuping, YANG Fan, WANG Guojian, DU Wei, GAO Bo, BORJIGIN Tenger. Microbial diversity above a shale gas field using high-throughput sequencing[J]. PETROLEUM GEOLOGY & EXPERIMENT, 2020, 42(3): 443-450. doi: 10.11781/sysydz202003443
Citation: GU Lei, XU Kewei, TANG Yuping, YANG Fan, WANG Guojian, DU Wei, GAO Bo, BORJIGIN Tenger. Microbial diversity above a shale gas field using high-throughput sequencing[J]. PETROLEUM GEOLOGY & EXPERIMENT, 2020, 42(3): 443-450. doi: 10.11781/sysydz202003443
shu

Microbial diversity above a shale gas field using high-throughput sequencing

doi: 10.11781/sysydz202003443
  • 1.

    Wuxi Research Institute of Petroleum Geology, SINOPEC, Wuxi, Jiangsu 214126, China

  • 2.

    Research Institute of Unconventional Resources, SINOPEC Petroleum Exploration & Production Research Institute, Beijing 100083, China

  • 3.

    Oil and Gas Survey, China Geological Survey, Beijing 100083, China

  • Received Date: 2020-01-27
  • Rev Recd Date: 2020-04-29
  • Publish Date: 2020-05-28
    Abstract
  • In recent years, microbial analysis has become a new detection technology in oil and gas surface exploration. The microbial diversity above a shale gas field was studied in order to further understand microbial information. High-throughput sequencing technology was used to study the abundance and diversity of hydrocarbon species in a shale gas field in the Dingshan area of the southeastern Sichuan Basin. Three main microorganisms were identified, including Methylophilales, Methylocystis, and Methylococcus, which are worthy of further biochemical research and can be used as potential oil and gas indicating bacteria. The developmental abundance of three kinds of microorganisms has a good matching relationship with the distribution of the fugitive area in Dingshan. The high-throughput analytical technology can be applied well in the study of oil and gas microorganisms in Dingshan area, and the distribution characteristics of microorganisms in bedrock samples were obtained. Furthermore, a preliminary analysis of the differences between oil and gas microbes in Dingshan and Jiaoshiba areas was made, indicating that the identified potential oil and gas indicating bacteria deserve further study.

     

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